Selecting specific PCR primers with MFEprimer.
نویسندگان
چکیده
Selecting specific primers is crucial for polymerase chain reaction (PCR). Nonspecific primers will bind to unintended genes and result in nonspecific amplicons. MFEprimer is a program for checking the specificity of PCR primers against the background DNA. In this chapter, we introduce: (1) the factors that affect the specificity of primers; (2) the principle of MFEprimer and its settings; (3) how to use the MFEprimer to examine the specificity of primers.
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ورودعنوان ژورنال:
- Methods in molecular biology
دوره 1275 شماره
صفحات -
تاریخ انتشار 2015